Presentations

Biochemistry

Description: Introduction to fluorescence and how it occurs, is measured, and how it's used

Keywords: fluorescent, labelling cellular structures, detecting biological molecules, measuring enzyme activity

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: An articulate presentation describing best practice for laboratory safety. It was designed for first year university undergraduates and details laboratory safety issues such as COSHH, hazard identification and risk management. There is then the opportunity to visit a 'virtual lab' where students are able to navigate Bob the Biochemist through a series of safety realted themes.

Keywords: Laboratory; Safety; Biochemistry; Biology; Hazard; COSHH; Risk management; Undergraduates; virtual laboratory

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: An articulate presentation detailing best practice for writing up a scientific laboratory book.

Keywords: Biology; Biochemistry; Reports; Writing; Experiments; Laboratory

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: An articulate presentation that explains to students new to the laboratory the best practice in writing up their practical experiments.

Keywords: Biology; Biochemistry; Reports; Writing; Experiments; Laboratory

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: An Articulate presentation on the structure and usage of micropipettes. Includes a video and quiz.

Keywords: Micropipettes; Pipettes; Measuring Liquids

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: This articulate presentation illustrates the basic principles of enzyme assays. The resource describes how to use spectrophotometry to measure reaction rates, the effect on substrate concentration on reaction rates and explains the principles behind the Michaelis-Menten equation.

Keywords: Enzymes; Biochemistry; Biology; Reaction; Rate; Substrate concentration; Michaelis-Menten; Spectrophotometry

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: Introduction to the three different types of inhibition; competitive, uncompetitive and non-competitive. An articulate presentation with quiz.

Keywords: Enzyme Inhibition; Inhibition Constant; quiz;

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: An articulate presentation explaining pH, the effective use of indicators and how to make up and use buffers correctly. It also contains a worked example of how to make up some standard buffer solutions.

Keywords: pH; pH meters; indicators; buffers; biochemistry

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: Brief multiple-choice quiz on protein separation, works well with the pH and Choosing and Designing Buffers presentation

Keywords: pH; pH meters; indicators; buffers; biochemistry

Authors: Dr Sue Bickerdike

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Description: Brief multiple-choice quiz on protein separation, works well with the pH and Choosing and Designing Buffers presentation

Keywords: pH; pH meters; indicators; buffers; biochemistry

Authors: Dr Sue Bickerdike

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Description: An Articulate presentation containing a comprehensive overview of molecular exclusion chromatography; including a video demonstration of how to set up a column.

Keywords: Molecular Exclusion Chromatography

Authors: Dr Sue Bickerdike

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Description: An articulate presentation describing the process of protein separation using ion exchange chromatography. This is illustrated using animations and a video demonstration of how to set up an ion exchange chromatography column.

Keywords: Biochemistry; Biology; Ion Exchange chromatography; pI; pH

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: An Articulate presentation containing an overview of affinity chromatography and a video demonstration of how to set up an affinity column.

Keywords: Affinity Chromatography

Authors: Dr Sue Bickerdike

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Description: Brief multiple-choice quiz on protein separation

Keywords: Chromatography; Protein Separation

Authors: Dr Sue Bickerdike

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Description: Brief multiple-choice quiz on protein separation

Keywords: Chromatography; Protein Separation

Authors: Dr Sue Bickerdike

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Description: Brief multiple-choice quiz on protein separation

Keywords: Chromatography; Protein Separation

Authors: Dr Sue Bickerdike

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Description: An articulate presentation detailing the theory and practice of protein separation using SDS PAGE. This unit includes a video demonstration of how to set up and run an SDS PAGE gel.

Keywords: Biology

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: Introduction thermodynamic measurement including determining activation energy, determining denaturation parameters, and working with van't Hoff plots. An Articulate presentation with examples.

Keywords: Thermodynamic Measurement; Activation Energy; Denaturation Parameters; van't Hoff Plots

Authors: Dr Sue Bickerdike

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Description: This articulate presentation describes some of the basic techniques in molecular biology with particular emphasis on the use of plasmids as cloning vectors. The theory behind the techniques used to isolate plasmid DNA, carrying out restriction digests, pcr and running agarose gels is described in detail, supported by animations to illustrate the text. Quizzes are interspersed within the body of the presentation to test the users understanding throughout.

Keywords: Biochemistry; Biology; Plasmids; restriction digest; DNA; electrophoresis; pcr; ethidium bromide; antibiotic resistance; origin of replication; cloning; vectors

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: An articulate presentation detailing the theory behind the use of spectrophotometers to detect and quantify biological molecules. Further to this the Beer-Lambert Law is explored and methods to establish standard curves for calibration and determination of unknown concentrations explained, as is the method for the determination of the peak wavelength of a sample. The presentation is supported by the use of animations and video demonstrations.

Keywords: Spectrophotometry; Analysis; Calibration; Standard Curve; Beer-Lambert; Peak wavelength; Biochemistry; Biology; molecules; video

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Description: A comprehensive Articulate presentation describing biochemical techniques used for molecular separation. Topics covered include pH and buffers, molecular exclusion chromatography, ion exchange chromatography, affinity chromatography and SDS PAGE. These techniques are described using both text and video demonstrations.

Keywords: Chromatography; Biochemistry; Ion exchange; affinity; SDS PAGE; Separation; pH; Buffers; molecular exclusion; video

Authors: Dr Sue Whittle, Dr Sue Bickerdike

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Microbiology

Description: An Articulate Engage interaction providing a comprehensive illustrated walk-through of the laboratory techniques required to analyse a faecal sample. Additionally there is a complete illustrated walk-through of the stages involved in the identification of a member of the genus bacillus. Finally, a walk-through showing the use of API 10 system for the identification of Enterobacteriaceae is included. A help file is provided and should be read first.

Keywords: Bacteria; Microbiology; Faeces; Faecal samples; Gram Stain; Catalase test; Pastorex; Bacillus; Enterobacteriaceae

Authors: Dr John Heritage, Dr Sue Bickerdike

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Description: An articulate presentation that includes several engage produced units that provide scripted photographic illustrations of microbiological techniques used in the culturing of bacteria. The elements included are: Dispensing a broth aseptically; Plating out a broth for single colonies; Assessing the degree of contamination in the laboratory; Separation of mixtures of bacteria; Inoculation of a broth culture. Each of these elements is contained as an individual unit in the zip file. Please note, these can be played on a web server, but can only be amended using the Articulate engage programme. Full instructions on their use are included in the zip file.

Keywords: ukoer

Authors: Dr John Heritage, Dr Sue Bickerdike

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Description: An Articulate Engage presentation covering the theory and practical assessment of: The Examination of milk (pasteurised vs unpasteurised) and Antimicrobial Susceptibility Testing. A help file is included and should be read first.

Keywords: Food Microbiology; Milk; Pasteurised; Unpasteurised; Susceptibility Testing; Antimicrobial; Microbiology

Authors: Dr John Heritage, Dr Sue Bickerdike

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Description: An Articulate presentation containing a series of short videos describing safe laboratory practice and how to dispose of various contaminated materials.

Keywords: Lab Safety; Contaminated; Disposal;

Authors: Dr John Heritage, Dr Sue Bickerdike

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Description: A demonstrative video on the set-up and use of the light microscope, along with annotated photographs of microscope parts

Keywords: Light Microscope; Microbiology

Authors: Dr John Heritage, Dr Sue Bickerdike

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Description: Illustrated walk-through of the essential chemostat techniques, including flow rates, sampling, and assays. An Articulate presentation. Note that the final screen refers to an offline practical.

Keywords: Chemostat; Flow Rate;Sample Preparation;Glucose Assay;Ammonia Assay

Authors: Dr Jeremy Knapp, Dr Sue Bickerdike

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Description: Illustrated walk-through of the essential chemostat techniques, including flow rates, sampling, and assays. An Articulate presentation. Note that the final screen refers to an offline practical.

Keywords: Chemostat; Flow Rate;Sample Preparation;Glucose Assay;Ammonia Assay

Authors: Dr Jeremy Knapp, Dr Sue Bickerdike

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Description: A series of Questionmark Perception files that use scenario-based projects to explain the theory of dilutions. Also included is a bank of questions requiring the reader to complete complex calculations related to dilution series and the construction of solutions to a specific concentration or molarity. The QTIXML file needs to be opened in QP Authoring Manager, converted to an assessment and exported into your own VLE.

Keywords: Dilutions; Serial dilutions; Microtitre; Microbiology; questions; calculations; molarity

Authors: Dr John Heritage, Dr Sue Bickerdike

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Description: A series of Articulate Engage presentations that use photographs and graphics to illustrate fungal structure. A guide on how to look at fungi using a light microscope is also included, as is a glossary of terms used in mycology.

Keywords: Fungi; Microbiology; Fungal structure; Light microscope; Glossary; Mycology

Authors: Dr John Heritage, Dr Sue Bickerdike

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Description: An articulate presentation that includes several engage produced units that provide scripted photographic illustrations of techniques used in virology. The techniques covered are: Plaque assay of Herpes Simplex virus; Neutralisation of virus infectivity as a means of virus identification; Assay of T4 Bacteriophage by the Agar Layer technique; Haemagglutination/Haemagglutination Inhibition test. Each of the engage interactions can be accessed individually if required once unzipped. A help file is included.

Keywords: Haemagglutination; Bacteriophage; Agar Layer technique; Herpes Simplex virus; Haemagglutination inhibition; Plaque assay; virus infectivity; virus identification; Microbiology

Authors: Dr John Heritage, Dr Sue Bickerdike

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Description: An articulate presentation that inlcudes two Articulate Engage presentation describing how to prepare a slide for staining and how to carry out a Gram stain. A help file is included and should be read first.

Keywords: medical microbiology; microbiology; Gram stain; bacteria

Authors: Dr John Heritage, Dr Sue Bickerdike

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Description: An Articulate Engage presentation that provides a detailed explanation of how to estimate the thermal death point of bacteria. The second unit included provides a walk-through illustration on the 'in-use' test to assess the efficacy of disinfectants. Both units are supported with photographs. A help guide is included and should be read first.

Keywords: Microbiology; Thermal Death Point; Bacteria; In Use Test; Disinfectants; Efficacy

Authors: Dr John Heritage, Dr Sue Bickerdike

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Sport and Exercise Science

Description: An introduction the practical laboratory aspects of biomechanics. Includes a complete walk-through of the equipment set-up, videography, data collection and processing, digitisation, and good scientific practice. (Note that the videos have sound but the presentation is not narrated)

Keywords: Biomechanics; Video Analysis; Kinematics; Experimental Set-up

Authors: Ms Daniella Strauss, Dr Sue Bickerdike

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